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KMID : 0387720080190010043
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Korean Journal of Blood Transfusion
2008 Volume.19 No. 1 p.43 ~ p.48
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Experience with the Automatic Blood Bank Instrument AutoVue Innova
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Lee Sun-Ho
Jeong Jeseph
Jeong Ui-Suk
Kim Min-Su
Jeong Yeong-Jin
Wee Jai-Ho
Kwon Seog-Woon
Kim Sung-Ryul
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Abstract
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Background:The AutoVue Innova (Ortho Clinical Diagnostic, Raritan, NJ, USA) is an automatic instrument for blood bank tests, and it has recently been introduced in Korea for the first time at our hospital. This instrument employs column agglutination technology and it performs blood bank tests automatically. We evaluated this instrument and we report on the results.
Methods:We performed ABO/RhD typing and antibody screening for 250 randomly selected samples, and crossmatching for 261 samples with using the AutoVue Innova in parallel with the conventional manual methods. For a sensitivity test, we added 3 samples of A2B3 and 2 samples of weak-D and serially diluted reagent antisera to the test pool and we measured turnaround time (TAT) for the antibody screening test.
Results:The concordance rates between AutoVue Innova and the manual methods for ABO/RhD blood typing, antibody screening and crossmatching tests were 99.6%, 100% and 98.9%, respectively. The overall retest rate was 0.5% and the main cause of the discrepancy was revealed to be hemolysis or an inadequate amount of the samples. The overall sensitivity of AutoVue Innova seems to be same as or better than the manual methods. The TAT for the antibody screening test was significantly shorter for the AutoVue Innova (64±43 min, n=512) than for the tube method (89±57 min, n=99) (P＜0.001).
Conclusion:The test results of AutoVue Innova were accurate and sensitive for the ABO/RhD typing, crossmatching and antibody screening tests. The TAT for the antibody screening test was remarkably shortened up to five times more samples could be tested without an increase of manpower.
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KEYWORD
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Automatic blood bank instrument, AutoVue Innova, Turnaround time
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